- Featured Product
- KD/KO Validated
SSRP1 Polyclonal antibody
SSRP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15696-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
Product Information
15696-1-AP targets SSRP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SSRP1 fusion protein Ag8318 相同性解析による交差性が予測される生物種 |
| Full Name | structure specific recognition protein 1 |
| Calculated molecular weight | 81 kDa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | BC005116 |
| Gene symbol | SSRP1 |
| Gene ID (NCBI) | 6749 |
| RRID | AB_2195756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| IHC protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| IF protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| IP protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Transl Med Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma. | ||
ACS Appl Mater Interfaces Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.
| ||
FASEB J Chronic exercise training activates histone turnover in mouse skeletal muscle fibers. | ||
Int J Med Sci SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway.
| ||
J Cell Mol Med SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.
|
