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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSR3 Polyclonal antibody (30851-1-AP)

    WB analysis using 30851-1-AP (same clone as 30851-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30851-1-AP (SSR3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30851-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SSR3 Polyclonal antibody (30851-1-AP)

    IHC staining of mouse brain using 30851-1-AP (same clone as 30851-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30851-1-AP (SSR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30851-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SSR3 Polyclonal antibody (30851-1-AP)

    IF Staining of mouse brain using 30851-1-AP (same clone as 30851-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SSR3 antibody (30851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30851-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30851-1-PBS targets SSR3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSR3 fusion protein Ag28130 相同性解析による交差性が予測される生物種
Full Name signal sequence receptor, gamma (translocon-associated protein gamma)
Calculated molecular weight 185 aa, 21 kDa
Observed molecular weight19 kDa
GenBank accession numberBC017203
Gene Symbol SSR3
Gene ID (NCBI) 6747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UNL2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mammalian TRAP is a heterotetrameric membrane protein complex which comprised of four membrane proteins/subunits: alpha, beta, gamma, and delta. SSR3 is also known as translocon-associated protein subunit gamma (TRAPG). The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. SSR3 assumes a central position in the mammalian TRAP complex, binding to the ribosome on the cytosolic face of the ER membrane and coordinating the remaining TRAP subunits with the ribosome and the other translocon components.