Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of A549 using 12212-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis using 12212-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12212-1-AP
WB result of SSBP1 antibody (12212-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSBP1 transfected A549 cells.
WB analysis of rat pancreas using 12212-1-AP
rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 12212-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12212-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 12212-1-AP
IP result of anti-SSBP1 (IP:12212-1-AP, 4ug; Detection:12212-1-AP 1:4000) with A375 cells lysate 1440 ug.
IHC staining of human ovary cancer using 12212-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12212-1-AP (SSBP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 12212-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12212-1-AP (SSBP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A375 using 12212-1-AP
Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12212-1-AP (SSBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SSBP1 Polyclonal antibody

SSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12212-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

Mt SSB, MtSSB, Mt-SSB, PWP1 interacting protein 17, PWP1-interacting protein 17

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (33)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse pancreas tissue, A375 cells, rat pancreas tissue, Caco-2 cells
Positive IP detected in	A375 cells
Positive IHC detected in	human stomach cancer tissue, human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A375 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 27 publications below
IHC	See 3 publications below
IF	See 5 publications below
ChIP	See 1 publications below

Product Information

12212-1-AP targets SSBP1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2860 Product name: Recombinant human SSBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-148 aa of BC000895 Sequence: MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE 相同性解析による交差性が予測される生物種
Full Name	single-stranded DNA binding protein 1
Calculated molecular weight	148 aa, 17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC000895
Gene Symbol	SSBP1
Gene ID (NCBI)	6742
RRID	AB_2195320
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q04837
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase

Protocols

Product Specific Protocols
IF protocol for SSBP1 antibody 12212-1-AP	Download protocol
IHC protocol for SSBP1 antibody 12212-1-AP	Download protocol
IP protocol for SSBP1 antibody 12212-1-AP	Download protocol
WB protocol for SSBP1 antibody 12212-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nature Stress response silencing by an E3 ligase mutated in neurodegeneration Authors - Diane L Haakonsen View Article
human,mouse	WB	Science Nucleotide metabolic rewiring enables NLRP3 inflammasome hyperactivation in obesity. Authors - Danhui Liu View Article
human	WB	Cell MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria. Authors - Aindrila Chatterjee View Article
human	WB	Cell MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels Authors - Luis Carlos Tábara View Article
human	WB	Cell Systematic identification of anticancer drug targets reveals a nucleus-to-mitochondria ROS-sensing pathway Authors - Junbing Zhang KD Validated View Article
human	IF	Nat Cell Biol Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal Authors - Laura E Newman View Article

Validation Data Gallery

WB analysis of A549 using 12212-1-AP

WB analysis using 12212-1-AP

WB analysis of A549 using 12212-1-AP

WB analysis of rat pancreas using 12212-1-AP

WB analysis of mouse pancreas using 12212-1-AP

WB analysis of A375 using 12212-1-AP

IP experiment of A375 using 12212-1-AP

IHC staining of human ovary cancer using 12212-1-AP

IHC staining of human stomach cancer using 12212-1-AP

IF Staining of A375 using 12212-1-AP

SSBP1 Polyclonal antibody

SSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Stress response silencing by an E3 ligase mutated in neurodegeneration

Nucleotide metabolic rewiring enables NLRP3 inflammasome hyperactivation in obesity.

MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.

MTFP1 controls mitochondrial fusion to regulate inner membrane quality control and maintain mtDNA levels

Systematic identification of anticancer drug targets reveals a nucleus-to-mitochondria ROS-sensing pathway

Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal

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