Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 14273-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRXN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14273-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 14273-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14273-1-AP (SRX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 14273-1-AP
IP result of anti-SRXN1 (IP:14273-1-AP, 4ug; Detection:14273-1-AP 1:1000) with A549 cells lysate 720 ug.
IHC staining of human lung cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14273-1-AP (SRX1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 14273-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 14273-1-AP (SRX1 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

SRXN1 Polyclonal antibody

SRXN1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14273-1-AP

Publications(36)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

C20orf139, EC:1.8.98.2, SRX, SRX1, Sulfiredoxin 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (36)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse liver tissue, Transfected HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells, U-251 cells
Positive IP detected in	A549 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 28 publications below
IHC	See 14 publications below
IF	See 2 publications below

Product Information

14273-1-AP targets SRXN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5613 Product name: Recombinant human SRX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-137 aa of BC047707 Sequence: MGLRAGGTLGRAGAGRGAPEGPGPSGGAQGGSIHSGRIAAVHNVPLSVLIRPLPSVLDPAKVQSLVDTIREDPDSVPPIDVLWIKGAQGGDYFYSFGGCHRYAAYQQLQRETIPAKLVQSTLSDLRVYLGASTPDLQ 相同性解析による交差性が予測される生物種
Full Name	sulfiredoxin 1 homolog (S. cerevisiae)
Calculated molecular weight	14 kDa
Observed molecular weight	14 kDa
GenBank accession number	BC047707
Gene Symbol	SRXN1
Gene ID (NCBI)	140809
RRID	AB_2195030
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BYN0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for SRXN1 antibody 14273-1-AP	Download protocol
IP protocol for SRXN1 antibody 14273-1-AP	Download protocol
WB protocol for SRXN1 antibody 14273-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Cancer Res Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer. Authors - Hartikainen Jaana M JM View Article
mouse		Nat Commun Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Authors - John T Olthoff View Article
		Blood Cancer J PMID: 27421095 Authors - K K Starheim View Article
mouse,human	WB,IHC	Cancer Lett Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis. Authors - Murli Mishra KD Validated View Article
human	WB,IHC	Proc Natl Acad Sci U S A Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling. Authors - Wei Qiou Q View Article
human	WB	Osteoarthritis Cartilage Age and oxidative stress regulate Nrf2 homeostasis in human articular chondrocytes Authors - E L Taylor View Article

Validation Data Gallery

WB analysis using 14273-1-AP

WB analysis of mouse liver using 14273-1-AP

WB analysis of Transfected HEK-293 using 14273-1-AP

IP experiment of A549 using 14273-1-AP

IHC staining of human lung cancer using 14273-1-AP

IHC staining of human lung cancer using 14273-1-AP

IHC staining of human kidney using 14273-1-AP

IHC staining of human kidney using 14273-1-AP

IHC staining of human breast cancer using 14273-1-AP

IHC staining of human breast cancer using 14273-1-AP

SRXN1 Polyclonal antibody

SRXN1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Genetic polymorphisms and protein expression of NRF2 and Sulfiredoxin predict survival outcomes in breast cancer.

Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle.

PMID: 27421095

Nrf2-activated expression of sulfiredoxin contributes to urethane-induced lung tumorigenesis.

Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.

Age and oxidative stress regulate Nrf2 homeostasis in human articular chondrocytes

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