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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRRT Polyclonal antibody (20353-1-AP)

    WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SRRT Polyclonal antibody (20353-1-AP)

    WB analysis of NIH/3T3 using 20353-1-AP (same clone as 20353-1-PBS)

    WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SRRT Polyclonal antibody (20353-1-AP)

    WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using SRRT Polyclonal antibody (20353-1-AP)

    IP experiment of Jurkat using 20353-1-AP (same clone as 20353-1-PBS)

    IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SRRT Polyclonal antibody (20353-1-AP)

    IF Staining of MCF-7 using 20353-1-AP (same clone as 20353-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SRRT Polyclonal antibody, PBS Only

SRRT Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 20353-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20353-1-PBS targets SRRT in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14196

Product name: Recombinant human SRRT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 527-876 aa of BC000082

Sequence: LIHTLDDRTQLWASEPGTPPLPTSLPSQNPILKNITDYLIEEVSAEEEELLGSSGGAPPEEPPKEGNPAEINVERDEKLIKVLDKLLLYLRIVHSLDYYNTCEYPNEDEMPNRCGIIHVRGPMPPNRISHGEVLEWQKTFEEKLTPLLSVRESLSEEEAQKMGRKDPEQEVEKFVTSNTQELGKDKWLCPLSGKKFKGPEFVRKHIFNKHAEKIEEVKKEVAFFNNFLTDAKRPALPEIKPAQPPGPAQILPPGLTPGLPYPHQTPQGLMPYGQPRPPILGYGAGAVRPAVPTGGPPYPHAPYGAGRGNYDAFRGQGGYPGKPRNRMVRGDPRAIVEYRDLDAPDDVDFF

相同性解析による交差性が予測される生物種
Full Name serrate RNA effector molecule homolog (Arabidopsis)
Calculated molecular weight 876 aa, 101 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC000082
Gene Symbol SRRT
Gene ID (NCBI) 51593
RRIDAB_3085603
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXP5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).

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