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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of mouse liver using 12822-1-AP (same clone as 12822-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis using 12822-1-AP (same clone as 12822-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of Jurkat using 12822-1-AP (same clone as 12822-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of UV treated HEK-293 using SRRM1 Polyclonal antibody (12822-1-AP)

    WB analysis of UV treated HEK-293 using 12822-1-AP (same clone as 12822-1-PBS)

    UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SRRM1 Polyclonal antibody (12822-1-AP)

    IP experiment of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

    IP result of anti-SRRM1 (IP:12822-1-AP, 4ug; Detection:12822-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SRRM1 Polyclonal antibody, PBS Only

SRRM1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12822-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12822-1-PBS targets SRRM1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3513

Product name: Recombinant human SRRM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC036187

Sequence: MDAGFFRGTSAEQDNRFSNKQKKLLKQLKFAECLEKKVDMSKVNLEVIKPWITKRVTEILGFEDDVVIEFIFNQLEVKNPDSKMMQINLTGFLNGKNAREFMGELWPLLLSAQENIAGIPSAFLELKKEEIKQRQIEQEKLASMKKQDEDKDKRDKEEKESSREKRERSHSPRRRKSRSPSPRRRSSPVRRERKRSHSRSPRHRTKSRSPSPAPEKKEKTPELPEPSVKVKEPSVQEATSTSDILKVPKPEPIPEPKEPSPEKNSKKEKEKEKTQPRSRSRSKSRSRTRSRSPSHTRPRRRHRSRSRSYSPRRRPSPRRRPSPRRRTPPRRMPPPPRHRRSRSPVRRRRR

相同性解析による交差性が予測される生物種
Full Name serine/arginine repetitive matrix 1
Calculated molecular weight 904 aa, 102 kDa
Observed molecular weight 120-160 kDa
GenBank accession numberBC036187
Gene Symbol SRRM1
Gene ID (NCBI) 10250
RRIDAB_2286613
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYB3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.

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