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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRP54 Recombinant antibody (84014-5-RR)

    WB analysis using 84014-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SRP54 Recombinant monoclonal antibody (84014-5-RR)

    WB analysis of MCF-7 using 84014-5-RR

    WB result of SRP54 antibody (84014-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP54 transfected MCF-7 cells.

  • Western Blot (WB) analysis of human placenta tissue using SRP54 Recombinant antibody (84014-5-RR)

    WB analysis of human placenta using 84014-5-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SRP54 Recombinant antibody (84014-5-RR)

    IF Staining of HeLa using 84014-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SRP54 antibody (84014-5-RR, Clone: 241125D6 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using SRP54 Recombinant antibody (84014-5-RR)

    FC experiment of HepG2 using 84014-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HeLa cells using SRP54 Recombinant antibody (84014-5-RR)

    FC experiment of HeLa using 84014-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84014-5-RR

    Affinity and Kinetic Characterization of 84014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-5-RR against Human SRP54 were performed. The affinity constant is 0.287 nM.

  • Featured Product
  • KD/KO Validated

SRP54 Recombinant monoclonal antibody

SRP54 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84014-5-RR
Clone No.241125D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241125D6, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84014-5-RR targets SRP54 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2327

Product name: Recombinant human SRP54 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 167-505 aa of BC003389

Sequence: DPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM

相同性解析による交差性が予測される生物種
Full Name signal recognition particle 54kDa
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
RRIDAB_3671583
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP61011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Protocols

Product Specific Protocols
IF protocol for SRP54 antibody 84014-5-RRDownload protocol
WB protocol for SRP54 antibody 84014-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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