Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, K-562 cells, Raji cells, mouse kidney tissue, mouse ovary tissue, mouse liver tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84061-3-RR targets SRP19 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SRP19 fusion protein Ag8903 相同性解析による交差性が予測される生物種 |
| Full Name | signal recognition particle 19kDa |
| Calculated molecular weight | 144 aa, 16 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC010947 |
| Gene Symbol | SRP19 |
| Gene ID (NCBI) | 6728 |
| RRID | AB_3671631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P09132 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRP19 antibody 84061-3-RR | Download protocol |
| IF protocol for SRP19 antibody 84061-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |