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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HL-60 cells using SRMS Polyclonal antibody (26447-1-AP)

    IP experiment of HL-60 using 26447-1-AP

    IP result of anti-SRMS (IP:26447-1-AP, 4ug; Detection:26447-1-AP 1:300) with HL-60 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRMS Polyclonal antibody (26447-1-AP)

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SRMS Polyclonal antibody (26447-1-AP)

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SRMS Polyclonal antibody

SRMS Polyclonal Antibody for IP, IHC, ELISA
Cat No. 26447-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Synonyms

C20orf148, SRM, SRMS, Tyrosine protein kinase Srms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHL-60 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26447-1-AP targets SRMS in IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24062

Product name: Recombinant human SRMS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-488 aa of BC153198

Sequence: EVFTYGQCPYEGMTNHETLQQIMRGYRLPRPAACPAEVYVLMLECWRSSPEERPSFATLREKLHAIHRCHP

相同性解析による交差性が予測される生物種
Full Name src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
Calculated molecular weight 488 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC153198
Gene Symbol SRMS
Gene ID (NCBI) 6725
RRIDAB_2880516
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3Y6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for SRMS antibody 26447-1-APDownload protocol
IP protocol for SRMS antibody 26447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols