Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 19858-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 19858-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 19858-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 19858-1-AP (Spermidine synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 19858-1-AP
IP result of anti-Spermidine synthase (IP:19858-1-AP, 4ug; Detection:19858-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human kidney using 19858-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19858-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody at dilution of 1:200 (under 40x lens).
IHC staining of human liver cancer using 19858-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19858-1-AP (Spermidine synthase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 19858-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Spermidine synthase antibody (19858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Spermidine synthase Polyclonal antibody

Spermidine synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19858-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SRM, SRML1, SPS1, SPDSY, PAPT

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, LNCaP cells, HepG2 cells, PC-3 cells, mouse liver tissue, rat liver tissue, RAW 264.7 cells, mouse brain tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human kidney tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 19 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

19858-1-AP targets Spermidine synthase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13936 Product name: Recombinant human SRM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-302 aa of BC000309 Sequence: MEPGPDGPAASGPAAIREGWFRETCSLWPGQALSLQVEQLLHHRRSRYQDILVFRSKTYGNVLVLDGVIQCTERDEFSYQEMIANLPLCSHPNPRKVLIIGGGDGGVLREVVKHPSVESVVQCEIDEDVIQVSKKFLPGMAIGYSSSKLTLHVGDGFEFMKQNQDAFDVIITDSSDPMGPAESLFKESYYQLMKTALKEDGVLCCQGECQWLHLDLIKEMRQFCQSLFPVVAYAYCTIPTYPSGQIGFMLCSKNPSTNFQEPVQPLTQQQVAQMQLKYYNSDVHRAAFVLPEFARKALNDVS 相同性解析による交差性が予測される生物種
Full Name	spermidine synthase
Calculated molecular weight	302 aa, 34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC000309
Gene Symbol	Spermidine synthase
Gene ID (NCBI)	6723
RRID	AB_10665555
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19623
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).

Protocols

Product Specific Protocols
IF protocol for Spermidine synthase antibody 19858-1-AP	Download protocol
IHC protocol for Spermidine synthase antibody 19858-1-AP	Download protocol
IP protocol for Spermidine synthase antibody 19858-1-AP	Download protocol
WB protocol for Spermidine synthase antibody 19858-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Spermine is an endogenous iron chelator that inhibits ferroptosis Authors - Man Li View Article
		Cell Polyamines buffer labile iron to suppress ferroptosis. Authors - Pushkal Sharma View Article
human,mouse,rat	WB,IF	Cell Metab Arachidonic acid triggers spermidine synthase secretion from primary tumor to induce skeletal muscle weakness upon irradiation Authors - Xiaohui Zhang View Article
human	WB	Cancer Res A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer Authors - Yanchao Yu KO Validated View Article
human	WB,IF	Nat Commun Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. Authors - Hongming Miao View Article
mouse	IF	J Nanobiotechnology Growth hormone-releasing peptide 6 (GHRP-6) hydrogel for acute kidney injury therapy via metabolic regulation. Authors - Xiaotong Zhao View Article

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Validation Data Gallery

WB analysis using 19858-1-AP

WB analysis using 19858-1-AP

WB analysis of LNCaP using 19858-1-AP

IP experiment of mouse liver using 19858-1-AP

IHC staining of human kidney using 19858-1-AP

IHC staining of human liver cancer using 19858-1-AP

IHC staining of human liver cancer using 19858-1-AP

IF Staining of HepG2 using 19858-1-AP

Spermidine synthase Polyclonal antibody

Spermidine synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Spermine is an endogenous iron chelator that inhibits ferroptosis

Polyamines buffer labile iron to suppress ferroptosis.

Arachidonic acid triggers spermidine synthase secretion from primary tumor to induce skeletal muscle weakness upon irradiation

A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer

Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.

Growth hormone-releasing peptide 6 (GHRP-6) hydrogel for acute kidney injury therapy via metabolic regulation.

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Validation Data Gallery

WB analysis using 19858-1-AP

WB analysis using 19858-1-AP

WB analysis of LNCaP using 19858-1-AP

IP experiment of mouse liver using 19858-1-AP

IHC staining of human kidney using 19858-1-AP

IHC staining of human liver cancer using 19858-1-AP

IHC staining of human liver cancer using 19858-1-AP

IF Staining of HepG2 using 19858-1-AP

Spermidine synthase Polyclonal antibody

Spermidine synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Spermine is an endogenous iron chelator that inhibits ferroptosis

Polyamines buffer labile iron to suppress ferroptosis.

Arachidonic acid triggers spermidine synthase secretion from primary tumor to induce skeletal muscle weakness upon irradiation

A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer

Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.

Growth hormone-releasing peptide 6 (GHRP-6) hydrogel for acute kidney injury therapy via metabolic regulation.

Able™

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