Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, HepG2 cells, human liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
16471-1-AP targets SRI in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9553 Product name: Recombinant human SRI protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-198 aa of BC011025 Sequence: MAYPGHPGAGGGYYPGGYGGAPGGPAFPGQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKPFNLETCRLMVSMLDRDMSGTMGFNEFKELWAVLNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQAVNSIAKRYSTNGKITFDDYIACCVKLRALTDSFRRRDTAQQGVVNFPYDDFIQCVMSV 相同性解析による交差性が予測される生物種 |
| Full Name | sorcin |
| Calculated molecular weight | 198 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC011025 |
| Gene Symbol | SRI |
| Gene ID (NCBI) | 6717 |
| RRID | AB_2255255 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRI antibody 16471-1-AP | Download protocol |
| IHC protocol for SRI antibody 16471-1-AP | Download protocol |
| IF protocol for SRI antibody 16471-1-AP | Download protocol |
| IP protocol for SRI antibody 16471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Physiol Targeting P-glycoprotein and SORCIN: Dihydromyricetin strengthens anti-proliferative efficiency of adriamycin via MAPK/ERK and Ca(2+) -mediated apoptosis pathways in MCF-7/ADR and K562/ADR. | ||
Arch Biochem Biophys Knockdown of sorcin increases HEI-OC1 cell damage induced by cisplatin in vitro. | ||
Cell Death Dis AnnexinA7 promotes epithelial-mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma. | ||
J Physiol Biochem Sorcin promotes proliferation of hepatocellular carcinoma by regulating VEGFA/B via PI3K pathway | ||
Int J Mol Sci Sorcin Inhibits Mitochondrial Apoptosis by Interacting with STAT3 via NF-κB Pathway |