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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SRFBP1 Polyclonal antibody (12792-1-AP)

    WB analysis of mouse heart using 12792-1-AP (same clone as 12792-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SRFBP1 Polyclonal antibody (12792-1-AP)

    WB analysis using 12792-1-AP (same clone as 12792-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SRFBP1 Polyclonal antibody (12792-1-AP)

    WB analysis of HeLa using 12792-1-AP (same clone as 12792-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SRFBP1 Polyclonal antibody (12792-1-AP)

    WB analysis of HepG2 using 12792-1-AP (same clone as 12792-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12792-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SRFBP1 Polyclonal antibody (12792-1-AP)

    IHC staining of human pancreas cancer using 12792-1-AP (same clone as 12792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12792-1-AP (SRFBP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12792-1-PBS in a different storage buffer formulation.

SRFBP1 Polyclonal antibody, PBS Only

SRFBP1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12792-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

BUD22, P49, p49/STRAP, Rlb1, Serum response factor-binding protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12792-1-PBS targets SRFBP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3517

Product name: Recombinant human SRFBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-429 aa of BC031222

Sequence: VAEVESSKNASEDNHSENTLYSNDNGSNLQREATVISEQKVKETKILAKKPIHNSKEKIAKMEHGPKAVTIANSPSKPSEKDSVVSLESQKTPADPKLKTLSQTKKNKGSDSSLSGNSDGGEEFCEEEKEYFDDSTEERFYKQSSMSEDSDSGDDFFIGKVRRTRKKESSCHSSVKEQKPLEKVFLKEDTGETHGDTRNDKIKPSTETRKLESVFFHSLSGSKSSRRNFKEQAPKTRSLDFPQNEPQIKNQFNKKLSGRLENTKQQLQLPLHPSWEASRRRKEQQSNIAVFQGKKITFDD

相同性解析による交差性が予測される生物種
Full Name serum response factor binding protein 1
Calculated molecular weight 429 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC031222
Gene Symbol SRFBP1
Gene ID (NCBI) 153443
RRIDAB_2877882
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEF9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SRFBP1, also named as P49, p49/STRAP and STRAP, is abundantly expressed in heart and skeletal muscle, and at much lower levels in brain and lung. It may be involved in regulating transcriptional activation of cardiac genes during the aging process. SRFBP1 may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human SRFBP1.

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