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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis of RAW 264.7 using 66742-1-Ig (same clone as 66742-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SRF Monoclonal antibody (66742-1-Ig)

    WB analysis of HeLa using 66742-1-Ig (same clone as 66742-1-PBS)

    WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRF Monoclonal antibody (66742-1-Ig)

    IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRF Monoclonal antibody (66742-1-Ig)

    IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51563-1

    Cytometric bead array sample test of MP51563-1

    Cytometric bead array sample test of MP51563-1, SRF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66742-1-PBS. Detection antibody: 66742-2-PBS.

  • Cytometric bead array standard curve of MP51563-1

    Cytometric bead array standard curve of MP51563-1

    Cytometric bead array standard curve of MP51563-1, SRF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66742-1-PBS. Detection antibody: 66742-2-PBS. Standard:Ag10386. Range: 0.391-50 ng/mL.

  • Featured Product
  • KD/KO Validated

SRF Monoclonal antibody, PBS Only (Capture)

SRF Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66742-1-PBS
Clone No.1G8F4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

1G8F4, MCM1, Serum response factor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66742-1-PBS targets SRF as part of a matched antibody pair:

MP51563-1: 66742-1-PBS capture and 66742-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10386

Product name: Recombinant human SRF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-348 aa of BC048211

Sequence: MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPG

相同性解析による交差性が予測される生物種
Full Name serum response factor (c-fos serum response element-binding transcription factor)
Calculated molecular weight 508 aa, 52 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC048211
Gene Symbol SRF
Gene ID (NCBI) 6722
RRIDAB_2882090
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11831
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).

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