• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FDFT1 Recombinant antibody

FDFT1 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230362C10

Cat no : 83020-1-RR

Synonyms

DGPT, ERG9, FDFT1, FPP:FPP farnesyltransferase, SQS, Squalene synthase, SS

Validation Data Gallery

Applications:
  • WB analysis using 83020-1-RR

    WB analysis using 83020-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83020-1-RR (FDFT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83020-1-RR

    FC experiment of U2OS using 83020-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human FDFT1 (83020-1-RR, Clone:230362C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inL02 cells, T-47D cells, SH-SY5Y cells, HepG2 cells, mouse testis tissue
Positive FC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83020-1-RR targets FDFT1 in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FDFT1 fusion protein Ag3743 相同性解析による交差性が予測される生物種
Full Name farnesyl-diphosphate farnesyltransferase 1
Calculated molecular weight 417 aa, 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC029641
Gene symbol FDFT1
Gene ID (NCBI) 2222
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SQS (Squalene synthase), also known as Farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).

Protocols

Product Specific Protocols
WB protocol for FDFT1 antibody 83020-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols