Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 17256-1-AP (same clone as 17256-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
WB analysis using 17256-1-AP (same clone as 17256-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 17256-1-AP (same clone as 17256-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 17256-1-AP (same clone as 17256-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 17256-1-AP (same clone as 17256-1-PBS)
IP result of anti-SQRDL (IP:17256-1-AP, 4ug; Detection:17256-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 17256-1-AP (same clone as 17256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17256-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SQRDL Polyclonal antibody, PBS Only

SQRDL Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 17256-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CGI 44, CGI-44, EC:1.8.5.8, SQOR, Sulfide dehydrogenase-like

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17256-1-PBS targets SQRDL in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10762 Product name: Recombinant human SQRDL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 100-450 aa of BC016836 Sequence: GRPTASVIPSGVEWIKARVTELNPDKNCIHTDDDEKISYRYLIIALGIQLDYEKIKGLPEGFAHPKIGSNYSVKTVEKTWKALQDFKEGNAIFTFPNTPVKCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYADALQEIIQERNLTVNYKKNLIEVRADKQEAVFENLDKPGETQVISYEMLHVTPPMSPPDVLKTSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKADLMPFLYWNMMLRGYWGGPAFLRKLFHLGMS 相同性解析による交差性が予測される生物種
Full Name	sulfide quinone reductase-like (yeast)
Calculated molecular weight	450 aa, 50 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC016836
Gene Symbol	SQRDL
Gene ID (NCBI)	58472
RRID	AB_2195894
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y6N5
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

WB analysis using 17256-1-AP (same clone as 17256-1-PBS)

WB analysis of HeLa using 17256-1-AP (same clone as 17256-1-PBS)

WB analysis of HepG2 using 17256-1-AP (same clone as 17256-1-PBS)

IP experiment of HeLa using 17256-1-AP (same clone as 17256-1-PBS)

IHC staining of human colon cancer using 17256-1-AP (same clone as 17256-1-PBS)

IHC staining of human liver cancer using 17256-1-AP (same clone as 17256-1-PBS)

IHC staining of mouse liver using 17256-1-AP (same clone as 17256-1-PBS)

IHC staining of rat liver using 17256-1-AP (same clone as 17256-1-PBS)

IHC staining of mouse brain using 17256-1-AP (same clone as 17256-1-PBS)

IHC staining of human testis using 17256-1-AP (same clone as 17256-1-PBS)