• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPZ1 Polyclonal antibody

SPZ1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18931-1-AP

Synonyms

NYD TSP1, spermatogenic leucine zipper 1, SPZ1, Testis specific protein 1, TSP1

Validation Data Gallery

Applications:
  • WB analysis of PC-3 using 18931-1-AP

    WB analysis of PC-3 using 18931-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 18931-1-AP

    WB analysis of DU 145 using 18931-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 18931-1-AP

    WB analysis of human testis using 18931-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18931-1-AP

    IHC staining of human testis using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 18931-1-AP

    IHC staining of human testis using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human skin using 18931-1-AP

    IHC staining of human skin using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 18931-1-AP

    IHC staining of human skin using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human brain using 18931-1-AP

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 18931-1-AP

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human spleen using 18931-1-AP

    IHC staining of human spleen using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 18931-1-AP

    IHC staining of human spleen using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human lung using 18931-1-AP

    IHC staining of human lung using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 18931-1-AP

    IHC staining of human lung using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human brain using 18931-1-AP

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 18931-1-AP

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human ovary using 18931-1-AP

    IHC staining of human ovary using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary using 18931-1-AP

    IHC staining of human ovary using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human kidney using 18931-1-AP

    IHC staining of human kidney using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 18931-1-AP

    IHC staining of human kidney using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of PC-3 using 18931-1-AP

    IF Staining of PC-3 using 18931-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 18931-1-AP (SPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, DU 145 cells, human testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18931-1-AP targets SPZ1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPZ1 fusion protein Ag6995 相同性解析による交差性が予測される生物種
Full Name spermatogenic leucine zipper 1
Calculated molecular weight 430 aa, 49 kDa
Observed molecular weight 49-55 kDa
GenBank accession numberBC033798
Gene symbol SPZ1
Gene ID (NCBI) 84654
RRIDAB_2195884
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SPZ1 antibody 18931-1-APDownload protocol
IHC protocol for SPZ1 antibody 18931-1-APDownload protocol
IF protocol for SPZ1 antibody 18931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols