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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTBN2 Polyclonal antibody (55107-1-AP)

    WB analysis using 55107-1-AP (same clone as 55107-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55107-1-AP (SPTBN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SPTBN2 Polyclonal antibody (55107-1-AP)

    WB analysis of human brain using 55107-1-AP (same clone as 55107-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 55107-1-AP (SPTBN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SPTBN2 Polyclonal antibody (55107-1-AP)

    IHC staining of human stomach cancer using 55107-1-AP (same clone as 55107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55107-1-AP (SPTBN2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SPTBN2 Polyclonal antibody (55107-1-AP)

    IHC staining of human stomach cancer using 55107-1-AP (same clone as 55107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55107-1-AP (SPTBN2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55107-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPTBN2 Polyclonal antibody, PBS Only

SPTBN2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55107-1-PBS targets SPTBN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name spectrin, beta, non-erythrocytic 2
Calculated molecular weight 271 kDa
Observed molecular weight 270 kDa
GenBank accession numberNM_006946
Gene Symbol SPTBN2
Gene ID (NCBI) 6712
RRIDAB_10804779
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15020
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPTBN2, also named as KIAA0302, belongs to the spectrin family. It probably plays an important role in neuronal membrane skeleton. Defects in SPTBN2 are the cause of spinocerebellar ataxia type 5 (SCA5). The antibody is specific to SPTBN2.

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