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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTAN1 Polyclonal antibody (31676-1-AP)

    WB analysis using 31676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31676-1-AP (SPTAN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPTAN1 Polyclonal antibody (31676-1-AP)

    WB analysis using 31676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31676-1-AP (SPTAN1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SPTAN1 Polyclonal antibody (31676-1-AP)

    IHC staining of human stomach cancer using 31676-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SPTAN1 Polyclonal antibody (31676-1-AP)

    IHC staining of mouse colon using 31676-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SPTAN1 Polyclonal antibody (31676-1-AP)

    IHC staining of mouse colon using 31676-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPTAN1 Polyclonal antibody (31676-1-AP)

    IF Staining of HeLa using 31676-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPTAN1 antibody (31676-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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SPTAN1 Polyclonal antibody

SPTAN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31676-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SPTA2, NEAS, Alpha II spectrin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, mouse brain tissue, HeLa cells, MDA-MB-231 cells, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31676-1-AP targets SPTAN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPTAN1 fusion protein Ag36472 相同性解析による交差性が予測される生物種
Full Name spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
Calculated molecular weight285kDa，2472aa
Observed molecular weight285 kDa,150 kDa
GenBank accession numberNM_003127.3
Gene Symbol SPTAN1
Gene ID (NCBI) 6709
RRIDAB_3670071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ13813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).

Protocols

Product Specific Protocols
WB protocol for SPTAN1 antibody 31676-1-APDownload protocol
IHC protocol for SPTAN1 antibody 31676-1-APDownload protocol
IF protocol for SPTAN1 antibody 31676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols