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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPRYD5 Polyclonal antibody

SPRYD5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20802-1-AP

Synonyms

SPRY domain containing 5, SPRYD5, TRIM51

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 20802-1-AP

    WB analysis of Jurkat using 20802-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20802-1-AP (SPRYD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 20802-1-AP

    WB analysis of rat brain using 20802-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20802-1-AP (SPRYD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20802-1-AP targets SPRYD5 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPRYD5 fusion protein Ag14723 相同性解析による交差性が予測される生物種
Full Name SPRY domain containing 5
Calculated molecular weight 293 aa, 34 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC005014
Gene symbol SPRYD5
Gene ID (NCBI) 84767
RRIDAB_2878743
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SPRYD5 antibody 20802-1-APDownload protocol
IHC protocol for SPRYD5 antibody 20802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols