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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using SPRY4 Polyclonal antibody (22765-1-AP)

    WB analysis of A375 using 22765-1-AP (same clone as 22765-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 22765-1-AP (SPRY4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SPRY4 Polyclonal antibody (22765-1-AP)

    IHC staining of mouse liver using 22765-1-AP (same clone as 22765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22765-1-AP (SPRY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22765-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SPRY4 Polyclonal antibody (22765-1-AP)

    IHC staining of mouse liver using 22765-1-AP (same clone as 22765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22765-1-AP (SPRY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22765-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SPRY4 Polyclonal antibody, PBS Only

SPRY4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22765-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Spry-4, Spry 4, sprouty homolog 4 (Drosophila), Protein sprouty homolog 4, HH17

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22765-1-PBS targets SPRY4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPRY4 fusion protein Ag18574 相同性解析による交差性が予測される生物種
Full Name sprouty homolog 4 (Drosophila)
Calculated molecular weight 299 aa, 33 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC125095
Gene Symbol SPRY4
Gene ID (NCBI) 81848
RRIDAB_2879163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C004
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPRY4, also named as Protein sprouty homolog 4, is a 299 amino acid protein, which contains one SPR (sprouty) domain and belongs to the sprouty family. SPRY4 Suppresses the INS receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. SPRY4 has a cellular function to regulate the actin cytoskeleton, independent of its inhibitory activity on the Ras/MAP kinase signalling. SPRY1, SPRY2, SPRY3, SPRY4, are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway.