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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW264.7 using SPRY3 Polyclonal antibody (17932-1-AP)

    WB analysis of RAW264.7 using 17932-1-AP (same clone as 17932-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 17932-1-AP (SPRY3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17932-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using SPRY3 Polyclonal antibody (17932-1-AP)

    WB analysis of COLO 320 using 17932-1-AP (same clone as 17932-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17932-1-AP (SPRY3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17932-1-PBS in a different storage buffer formulation.

SPRY3 Polyclonal antibody, PBS Only

SPRY3 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 17932-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

HSPRY3, Protein sprouty homolog 3, sprouty homolog 3 (Drosophila), Sprouty RTK signaling antagonist 3, Sprouty3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17932-1-PBS targets SPRY3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12364

Product name: Recombinant human SPRY3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-218 aa of BC074973

Sequence: MDAAVTDDFQQILPIEQLRSTHASNDYVERPPAPCKQALSSPSLIVQTHKSDWSLATMPTSLPRSLSQCHQLQPLPQHLSQSSIASSMSHSTTASDQRLLASITPSPSGQSIIRTQPGAGVHPKADGALKGEAEQSAGHPSEHLFICEECGRCKCVPCTAARPLPSCWLCNQRCLCSAESLLDYGTCLCCVKGLFYHCSTDDEDNCADEPCSCGPSSC

相同性解析による交差性が予測される生物種
Full Name sprouty homolog 3 (Drosophila)
Calculated molecular weight 288 aa, 31 kDa
Observed molecular weight 31-33 kDa
GenBank accession numberBC074973
Gene Symbol SPRY3
Gene ID (NCBI) 10251
RRIDAB_2195432
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43610
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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