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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SPRR3 Polyclonal antibody (11742-1-AP)

    WB analysis of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using SPRR3 Polyclonal antibody (11742-1-AP)

    WB analysis of human liver using 11742-1-AP (same clone as 11742-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SPRR3 Polyclonal antibody (11742-1-AP)

    IP experiment of COLO 320 using 11742-1-AP (same clone as 11742-1-PBS)

    IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPRR3 Polyclonal antibody (11742-1-AP)

    IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPRR3 Polyclonal antibody (11742-1-AP)

    IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPRR3 Polyclonal antibody (11742-1-AP)

    IHC staining of human breast cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPRR3 Polyclonal antibody (11742-1-AP)

    IHC staining of human breast cancer using 11742-1-AP (same clone as 11742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPRR3 Polyclonal antibody (11742-1-AP)

    IF Staining of HeLa using 11742-1-AP (same clone as 11742-1-PBS)

    Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPRR3 Polyclonal antibody, PBS Only

SPRR3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11742-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11742-1-PBS targets SPRR3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2337

Product name: Recombinant human SPRR3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-161 aa of BC017802

Sequence: MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKVPEPCPSTVTPGPAQQKTKQK

相同性解析による交差性が予測される生物種
Full Name small proline-rich protein 3
Calculated molecular weight 19 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC017802
Gene Symbol SPRR3
Gene ID (NCBI) 6707
RRIDAB_2195272
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBC9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.

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