Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 16822-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 16822-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16822-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 16822-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16822-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 16822-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 16822-1-AP
IP result of anti-SPR (IP:16822-1-AP, 4ug; Detection:16822-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human liver cancer using 16822-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16822-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 16822-1-AP
Immunofluorescent analysis of Hela cells, using SPR antibody 16822-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SPR Polyclonal antibody

SPR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16822-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:1.1.1.153, SDR38C1, Sepiapterin reductase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, rat heart tissue, rat liver tissue, human liver tissue, K-562 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

16822-1-AP targets SPR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10395 Product name: Recombinant human SPR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC017310 Sequence: MEGGLGRAVCLLTGASRGFGRTLAPLLASLLSPGSVLVLSARNDEALRQLEAELGAERSGLRVVRVPADLGAEAGLQQLLGALRELPRPKGLQRLLLINNAGSLGDVSKGFVDLSDSTQVNNYWALNLTSMLCLTSSVLKAFPDSPGLNRTVVNISSLCALQPFKGWALYCAGKAARDMLFQVLALEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFYDK 相同性解析による交差性が予測される生物種
Full Name	sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
Calculated molecular weight	261 aa, 28 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC017310
Gene Symbol	SPR
Gene ID (NCBI)	6697
RRID	AB_2286632
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35270
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for SPR antibody 16822-1-AP	Download protocol
IHC protocol for SPR antibody 16822-1-AP	Download protocol
IP protocol for SPR antibody 16822-1-AP	Download protocol
WB protocol for SPR antibody 16822-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Chem Biol Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers. Authors - Mariluz Soula View Article
mouse	WB	Acta Physiol (Oxf) Tetrahydrobiopterin synthesis and metabolism is impaired in dystrophin-deficient mdx mice and humans. Authors - Angus Lindsay View Article
	WB	Cell Death Dis Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator Authors - Masamitsu Sone View Article
human	WB	Phytomedicine Cimifugin suppresses type 2 airway inflammation by binding to SPR and regulating its protein expression in a non-enzymatic manner Authors - Xiaoqun Gu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16822-1-AP

WB analysis of rat heart using 16822-1-AP

WB analysis of K-562 using 16822-1-AP

WB analysis of human liver using 16822-1-AP

WB analysis of HepG2 using 16822-1-AP

WB analysis of rat liver using 16822-1-AP

IP experiment of mouse liver using 16822-1-AP

IHC staining of human liver cancer using 16822-1-AP

IHC staining of human liver cancer using 16822-1-AP

IF Staining of HeLa using 16822-1-AP

SPR Polyclonal antibody

SPR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.

Tetrahydrobiopterin synthesis and metabolism is impaired in dystrophin-deficient mdx mice and humans.

Identification of genes supporting cold resistance of mammalian cells: lessons from a hibernator

Cimifugin suppresses type 2 airway inflammation by binding to SPR and regulating its protein expression in a non-enzymatic manner