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Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of human colon using 13255-1-AP (same clone as 13255-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of mouse colon using 13255-1-AP (same clone as 13255-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of Jurkat using 13255-1-AP (same clone as 13255-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of Jurkat using 13255-1-AP (same clone as 13255-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of human colon using 13255-1-AP (same clone as 13255-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human kidney using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human kidney using 13255-1-AP (same clone as 13255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13255-1-PBS in a different storage buffer formulation.

SPPL2A Polyclonal antibody, PBS Only

SPPL2A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13255-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13255-1-PBS targets SPPL2A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3935

Product name: Recombinant human SPPL2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 361-520 aa of BC025740

Sequence: ITKNGESIMVELAAGPFGNNEKLPVVIRVPKLIYFSVMSVCLMPVSILGFGDIIVPGLLIAYCRRFDVQTGSSYIYYVSSTVAYAIGMILTFVVLVLMKKGQPALLYLVPCTLITASVVAWRRKEMKKFWKGNSYQMMDHLDCATNEENPVISGEQIVQQ

相同性解析による交差性が予測される生物種
Full Name signal peptide peptidase-like 2A
Calculated molecular weight 520 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC025740
Gene Symbol SPPL2A
Gene ID (NCBI) 84888
RRIDAB_2195000
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCT8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPPL2A(Signal peptide peptidase-like 2A) is also named as IMP3(Intramembrane protease 3), PSL2(Presenilin-like protein 2) and belongs to the peptidase A22B family. It is an aspartyl intramembrane protease, has been implicated in the proteolysis of TNF-alpha, Fas Ligand and Bri2 and endogenous signal-peptide-peptidase-like2A (SPPL2a) is localised in lysosomes/late endosomes(PMID:21896273). This protein has a signal peptide with 25 amino acid and seven glycosylation sites.

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