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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPP2 Polyclonal antibody (30922-1-AP)

    WB analysis using 30922-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30922-1-AP (SPP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SPP2 Polyclonal antibody

SPP2 Polyclonal Antibody for WB, ELISA
Cat No. 30922-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SPP24, Spp 24, Secreted phosphoprotein 24, Secreted phosphoprotein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30922-1-AP targets SPP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPP2 fusion protein Ag29299 相同性解析による交差性が予測される生物種
Full Name secreted phosphoprotein 2, 24kDa
Calculated molecular weight 24 kDa
Observed molecular weight24 kDa
GenBank accession numberNM_006944
Gene Symbol SPP2
Gene ID (NCBI) 6694
RRIDAB_3669785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13103
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPP2 (Secreted phosphoprotein 24), also known as SPP24. SPP2 is a secreted phosphoprotein, which was originally isolated and purified from bovine bone density. It is widely distributed in the body, mainly produced in the liver and transported to other tissues. SPP2 is expressed in many tissues, especially in the liver. Studies have shown that the expression level of SPP2 is up-regulated in liver cirrhosis. In addition, the expression of SPP2 is significantly related to many genes, including APOA1, SLC2A2 and TTR (PMID: 38448371). SPP2 has many biological functions, including: it can inhibit the activity of cysteine protease, participate in bone formation, inhibit calcification, and regulate BMP/TGF-β signaling pathway. SPP2 may be a tumor suppressor, which inhibits the development of HCC by inhibiting BMP/TGF-β signaling pathway. The molecular weight of SPP2 is 24 kDa.

Protocols

Product Specific Protocols
WB protocol for SPP2 antibody 30922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols