Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, HEK-293 cells, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
22243-1-AP targets SPOCD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17734 Product name: Recombinant human SPOCD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 854-1204 aa of BC114430 Sequence: PAAPTKALPCLPPWEGVLDMFSIKRFRARAQLVSGHSCRLVQALPTVIRSAGCIPSNIVWDLLASICPAKAKDVCVVRLCPHGARDTQNCRLLYSYLNDRQRHGLASVEHMGMVLLPLPAFQPLPTRLRPLGGPGLEVTHSSLLLAVLLPKEGLPDTAGSSPWLGKVQKMVSFNSKVEKRYYQPDDRRPNVPLKGTPPPGGAWQQSQGRGSIAPRGISAWQRPPRGRGRLWPEPENWQHPGRGQWPPEPGLRQSQHPYSVAPAGHGFGRGQHFHRDSCPHQALLRHLESLATMSHQLQALLCPQTKSSIPRPLQRLSSALAAPEPPGPARDSSLGPTDEAGSECPFPRKA 相同性解析による交差性が予測される生物種 |
| Full Name | SPOC domain containing 1 |
| Calculated molecular weight | 1203 aa, 129 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC114430 |
| Gene Symbol | SPOCD1 |
| Gene ID (NCBI) | 90853 |
| RRID | AB_2879045 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q6ZMY3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PIWI protein MIWI2 (PIWIL4; 610315) and its associated PIWI-interacting RNAs (piRNAs) direct DNA methylation of transposable elements in the male germline. SPOCD1 is an essential nuclear effector of MIWI2- and piRNA-directed DNA methylation (PubMed: 32674113).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPOCD1 antibody 22243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Discov IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A | ||
Oncotarget Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma. | ||
World J Surg Oncol SPOCD1 expression in clear cell renal cell carcinoma: its implications for the prognosis and immunotherapy. |