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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPOCD1 Polyclonal antibody

SPOCD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22243-1-AP

Synonyms

RP11 84A19.1, SPOC domain containing 1, SPOCD1

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 22243-1-AP

    WB analysis of SH-SY5Y using 22243-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 22243-1-AP

    WB analysis of human testis using 22243-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 22243-1-AP

    WB analysis of HEK-293 using 22243-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

22243-1-AP targets SPOCD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPOCD1 fusion protein Ag17734 相同性解析による交差性が予測される生物種
Full Name SPOC domain containing 1
Calculated molecular weight 1203 aa, 129 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC114430
Gene symbol SPOCD1
Gene ID (NCBI) 90853
RRIDAB_2879045
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PIWI protein MIWI2 (PIWIL4; 610315) and its associated PIWI-interacting RNAs (piRNAs) direct DNA methylation of transposable elements in the male germline. SPOCD1 is an essential nuclear effector of MIWI2- and piRNA-directed DNA methylation (PubMed: 32674113).

Protocols

Product Specific Protocols
WB protocol for SPOCD1 antibody 22243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.

Authors - Takashi Sakaguchi
View Article