Validation Data Gallery

Filter: AllWBIHCFC (Intra)

WB analysis using 16110-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 16110-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16110-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16110-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 16110-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 16110-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16110-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 16110-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 16110-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16110-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 16110-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 16110-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 10x lens).
IHC staining of human skin cancer using 16110-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 40x lens).
FC experiment of HepG2 using 16110-1-AP
(blue)1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PBK (16110-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.8 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Featured Product
KD/KO Validated

PBK/SPK Polyclonal antibody

PBK/SPK Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 16110-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Synonyms

PBK, Cancer/testis antigen 84, CT84, EC:2.7.12.2, Lymphokine-activated killer T-cell-originated protein kinase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, mouse ovary tissue, mouse testis tissue, NIH/3T3 cells, rat testis tissue
Positive IHC detected in	human placenta tissue, human lung cancer tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:3000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 3 publications below
IF	See 3 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

16110-1-AP targets PBK/SPK in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9033 Product name: Recombinant human SPK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-322 aa of BC015191 Sequence: MEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV 相同性解析による交差性が予測される生物種
Full Name	PDZ binding kinase
Calculated molecular weight	322 aa, 36 kDa
Observed molecular weight	36-40 kDa
GenBank accession number	BC015191
Gene Symbol	PBK
Gene ID (NCBI)	55872
RRID	AB_2283508
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96KB5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).

Protocols

Product Specific Protocols
IHC protocol for PBK/SPK antibody 16110-1-AP	Download protocol
WB protocol for PBK/SPK antibody 16110-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Genomics Proteomics Bioinformatics Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma. Authors - Yixian Cun View Article
mouse	WB,IF	FASEB J Autophagy plays a positive role in induction of epidermal proliferation. Authors - Junyi Wang View Article
mouse	IF	Invest Ophthalmol Vis Sci Single-Cell Transcriptomics Reveals Cellular Heterogeneity and Complex Cell-Cell Communication Networks in the Mouse Cornea Authors - Yueh-Feng Wu View Article
human	IHC	Biomolecules Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods Authors - Haofuzi Zhang View Article
mouse	WB,IF	Invest Ophthalmol Vis Sci Single-Cell RNA Transcriptome Helps Define the Limbal/Corneal Epithelial Stem/Early Transit Amplifying Cells and How Autophagy Affects This Population. Authors - Nihal Kaplan KD Validated View Article
rat	WB	Front Endocrinol (Lausanne) PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas. Authors - Kejing Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16110-1-AP

WB analysis using 16110-1-AP

WB analysis of mouse testis using 16110-1-AP

WB analysis of Jurkat using 16110-1-AP

WB analysis of NIH/3T3 using 16110-1-AP

WB analysis of mouse ovary using 16110-1-AP

WB analysis of HeLa using 16110-1-AP

IHC staining of human placenta using 16110-1-AP

IHC staining of human placenta using 16110-1-AP

IHC staining of human lung cancer using 16110-1-AP

IHC staining of human lung cancer using 16110-1-AP

IHC staining of human skin cancer using 16110-1-AP

IHC staining of human skin cancer using 16110-1-AP

FC experiment of HepG2 using 16110-1-AP

PBK/SPK Polyclonal antibody

PBK/SPK Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.

Autophagy plays a positive role in induction of epidermal proliferation.

Single-Cell Transcriptomics Reveals Cellular Heterogeneity and Complex Cell-Cell Communication Networks in the Mouse Cornea

Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods

Single-Cell RNA Transcriptome Helps Define the Limbal/Corneal Epithelial Stem/Early Transit Amplifying Cells and How Autophagy Affects This Population.

PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas.