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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    WB analysis of mouse brain using 10107-2-AP (same clone as 10107-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10107-2-AP (SPIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10107-2-AP (SPIN2B antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human colon using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPIN2B Polyclonal antibody (10107-2-AP)

    IF Staining of HeLa using 10107-2-AP (same clone as 10107-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPIN2B Polyclonal antibody (10107-2-AP)

    IF Staining of HEK-293 using 10107-2-AP (same clone as 10107-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

SPIN2B Polyclonal antibody, PBS Only

SPIN2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10107-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10107-2-PBS targets SPIN2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0157

Product name: Recombinant human SPIN2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 35-223 aa of BC000044

Sequence: KKQRGRPSSQPRRNIVGCRISHGWKEGDEPITQWKGTVLDQVPINPSLYLVKYDGIDCVYGLELHRDERVLSLKILSDRVASSHISDANLANTIIGKAVEHMFEGEHGSKDEWRGMVLAQAPIMKAWFYITYEKDPVLYMYQLLDDYKEGDLRIMPESSESPPTEREPGGVVDGLIGKHVEYTKEDGSK

相同性解析による交差性が予測される生物種
Full Name spindlin family, member 2B
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC000044
Gene Symbol SPIN2B
Gene ID (NCBI) 474343
RRIDAB_2196120
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPZ2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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