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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPIB Polyclonal antibody

SPIB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15768-1-AP

Synonyms

SPI B, SPIB, Transcription factor Spi B

Validation Data Gallery

Applications:
  • WB analysis of L02 using 15768-1-AP

    WB analysis of L02 using 15768-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15768-1-AP (SPIB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 15768-1-AP

    IP experiment of L02 using 15768-1-AP

    IP result of anti-SPIB (IP:15768-1-AP, 4ug; Detection:15768-1-AP 1:300) with L02 cells lysate 1800ug.

  • IHC staining of human liver cancer using 15768-1-AP

    IHC staining of human liver cancer using 15768-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPIB antibody included
  • IHC staining of human liver cancer using 15768-1-AP

    IHC staining of human liver cancer using 15768-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPIB antibody included

Tested Applications

Positive WB detected inL02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SPIB Ready-To-Use IHC Kit. SPIB primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15768-1-AP targets SPIB in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPIB fusion protein Ag8404 相同性解析による交差性が予測される生物種
Full Name Spi-B transcription factor (Spi-1/PU.1 related)
Calculated molecular weight 262 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC007921
Gene symbol SPIB
Gene ID (NCBI) 6689
RRIDAB_2878182
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SPIB antibody 15768-1-APDownload protocol
IHC protocol for SPIB antibody 15768-1-APDownload protocol
IP protocol for SPIB antibody 15768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells.

Authors - Xunping Zhao
View Article