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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis of A549 using 17096-1-AP (same clone as 17096-1-PBS)

    WB result of SPHK2 antibody (17096-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPHK2 transfected A549 cells. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis using 17096-1-AP (same clone as 17096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    WB analysis of human kidney using 17096-1-AP (same clone as 17096-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17096-1-AP (SPHK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IP experiment of mouse liver using 17096-1-AP (same clone as 17096-1-PBS)

    IP result of anti-SPHK2 (IP:17096-1-AP, 4ug; Detection:17096-1-AP 1:800) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human breast cancer using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human breast cancer using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human kidney using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human kidney using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of mouse kidney using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of rat kidney using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of rat small intestine using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17096-1-AP (SPHK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human liver using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SPHK2 Polyclonal antibody (17096-1-AP)

    IHC staining of human liver using 17096-1-AP (same clone as 17096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 17096-1-AP (SPHK2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPHK2 Polyclonal antibody (17096-1-AP)

    IF Staining of HeLa using 17096-1-AP (same clone as 17096-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPHK2 antibody (17096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SPHK2 Polyclonal antibody (17096-1-AP)

    FC experiment of HeLa using 17096-1-AP (same clone as 17096-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SPHK2 (17096-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17096-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17096-1-PBS targets SPHK2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPHK2 fusion protein Ag10569 相同性解析による交差性が予測される生物種
Full Name sphingosine kinase 2
Calculated molecular weight 618 aa, 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC010671
Gene Symbol SPHK2
Gene ID (NCBI) 56848
RRIDAB_10598479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRA0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).