Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, MCF-7 cells，MDA-MB-231 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 76 publications below |
| IHC | See 21 publications below |
| IF | See 13 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
10670-1-AP targets SPHK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1051 Product name: Recombinant human SPHK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 85-384 aa of BC008040 Sequence: HEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL 相同性解析による交差性が予測される生物種 |
| Full Name | sphingosine kinase 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC008040 |
| Gene Symbol | SPHK1 |
| Gene ID (NCBI) | 8877 |
| RRID | AB_2195809 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| IHC protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| IP protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| WB protocol for SPHK1 antibody 10670-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Targeting SPHK1 in macrophages remodels the tumor microenvironment and enhances anti-PD-1 immunotherapy efficacy in colorectal cancer liver metastasis. | ||
Nat Aging Modulating mTOR-dependent astrocyte substate transitions to alleviate neurodegeneration | ||
Autophagy Mitophagy-mediated S1P facilitates muscle adaptive responses to endurance exercise through SPHK1-S1PR1/S1PR2 in slow-twitch myofibers
| ||
Redox Biol YTHDF1 mitigates acute kidney injury via safeguarding m6A-methylated mRNAs in stress granules of renal tubules | ||
Nat Cardiovasc Res Sphingosine kinase 1 is integral for elastin deficiency-induced arterial hypermuscularization. | ||
Cell Death Dis POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling. |