Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19815-1-AP targets SPG21 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13851 Product name: Recombinant human SPG21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-308 aa of BC000244 Sequence: MGEIKVSPDYNWFRGTVPLKKIIVDDDDSKIWSLYDAGPRSIRCPLIFLPPVSGTADVFFRQILALTGWGYRVIALQYPVYWDHLEFCDGFRKLLDHLQLDKVHLFGASLGGFLAQKFAEYTHKSPRVHSLILCNSFSDTSIFNQTWTANSFWLMPAFMLKKIVLGNFSSGPVDPMMADAIDFMVDRLESLGQSELASRLTLNCQNSYVEPHKIRDIPVTIMDVFDQSALSTEAKEEMYKLYPNARRAHLKTGGNFPYLCRSAEVNLYVQIHLLQFHGTKYAAIDPSMVSAEELEVQKGSLGISQEEQ 相同性解析による交差性が予測される生物種 |
| Full Name | spastic paraplegia 21 (autosomal recessive, Mast syndrome) |
| Calculated molecular weight | 308 aa, 35 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC000244 |
| Gene Symbol | SPG21 |
| Gene ID (NCBI) | 51324 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZD8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPG21, also known as Maspardin, is a protein encoded by the SPG21 gene on human chromosome 15q22.31. SPG21 is critical for the proper movement and positioning of lysosomes within neurons, which is essential for maintaining axonal health. Mutations in the SPG21 gene underlie hereditary spastic paraplegia (SPG) type 21 (also known as Mast syndrome). This is a complex form of SPG characterized by motor coordination defects due to axonal degeneration of corticospinal neurons and associated with dementia, cerebellar, and extrapyramidal abnormalities and, in some cases, seizures.(PMID: 41400694,PMID: 34492745)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPG21 antibody 19815-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |