Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, PANC-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 16 publications below |
| IHC | See 2 publications below |
| IF | See 7 publications below |
| IP | See 1 publications below |
Product Information
13791-1-AP targets Spartin/SPG20 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4815 Product name: Recombinant human Spartin, SPG20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC047083 Sequence: MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSKESEHTGTGWESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQHAEVNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEEFYRNHSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTAGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQI 相同性解析による交差性が予測される生物種 |
| Full Name | spastic paraplegia 20 (Troyer syndrome) |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 75-84 kDa |
| GenBank accession number | BC047083 |
| Gene Symbol | Spartin |
| Gene ID (NCBI) | 23111 |
| RRID | AB_2195683 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N0X7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Spartin/SPG20 antibody 13791-1-AP | Download protocol |
| IHC protocol for Spartin/SPG20 antibody 13791-1-AP | Download protocol |
| IP protocol for Spartin/SPG20 antibody 13791-1-AP | Download protocol |
| WB protocol for Spartin/SPG20 antibody 13791-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Host Microbe A CRISPR activation screen identifies SPART as a pan-orthoflavivirus restriction factor. | ||
Nat Cell Biol The Troyer syndrome protein spartin mediates selective autophagy of lipid droplets | ||
Autophagy Lipophagy fuels phosphatidylcholine synthesis for Newcastle disease virus replication. | ||
Nat Commun Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2 | ||
Adv Sci (Weinh) Targeting Endogenous Lipophagy: A Novel Strategy to Enhance MSC Osteogenesis and Mineralization for Senile Osteoporosis Therapy.
| ||
Proc Natl Acad Sci U S A Spartin-mediated lipid transfer facilitates lipid droplet turnover |