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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of mouse testis using 17826-1-AP (same clone as 17826-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

    WB result of SPERT antibody (17826-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPERT transfected PC-3 cells. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spermatophore tissue using SPERT Polyclonal antibody (17826-1-AP)

    WB analysis of mouse spermatophore using 17826-1-AP (same clone as 17826-1-PBS)

    mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SPERT Polyclonal antibody (17826-1-AP)

    IF Staining of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SPERT antibody (17826-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPERT Polyclonal antibody, PBS Only

SPERT Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 17826-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17826-1-PBS targets SPERT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12200

Product name: Recombinant human SPERT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-412 aa of BC027609

Sequence: MQPEGLKCRMEESNAERGTAEPFPRLHNLYSTPRCAQQAALPRLSRRMASQHSYPLNRFSSVPLDPMERPMSQADLELDYNPPRVQLSDEMFVFQDGRWVNENCRLQSPYFSPSASFHHKLHHKRLAKECMLQEENKSLREENKALREENRMLSKENKILQVFWEEHKASLGREESRAPSPLLHKDSASLEVVKKDHVALQVPRGKEDSTLQLLREENRALQQLLEQKQAYWAQAEDTAAPAEESKPAPSPHEEPCSPGLLQDQGSGLSSRFEEPKGPPARQEDSKELRALRKMVSNMSGPSGEEEAKVGPGLPDGCQPLQLLREMRQALQALLKENRLLQEENRTLQVLRAEHRGFQEENKALWENNKLKLQQKLVIDTVTEVTARMEMLIEELYAFMPARSQDPKKPSRV

相同性解析による交差性が予測される生物種
Full Name spermatid associated
Calculated molecular weight 448 aa, 47 kDa, 52 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC027609
Gene Symbol SPERT
Gene ID (NCBI) 220082
RRIDAB_2195674
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NA61
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]

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