Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse skeletal muscle tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18716-1-AP targets SPEG in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | SPEG complex locus |
| Calculated molecular weight | 354 kDa |
| GenBank accession number | NM_005876 |
| Gene Symbol | SPEG |
| Gene ID (NCBI) | 10290 |
| RRID | AB_3085566 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15772 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPEG, also named as BPEG, APEG1 and KIAA1297, is highly similar to the corresponding rat and mouse proteins. Expression of SPEG is thought to serve as a marker for differentiated vascular smooth muscle cells which may have a role in regulating growth and differentiation of this cell type. SPEG is expressed in vascular smooth muscle cells and is down-regulated by vascular injury. Multiple transcripts are produced from the SPEG complex locus by alternative splicing and alternative promoter usage. The antibody recognizes the C-term of SPEG.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPEG antibody 18716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |



