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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPEF2 Polyclonal antibody (27989-1-AP)

    WB analysis using 27989-1-AP (same clone as 27989-1-PBS)

    Rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 27989-1-AP (SPEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27989-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat testis tissue using SPEF2 Polyclonal antibody (27989-1-AP)

    IP experiment of rat testis using 27989-1-AP (same clone as 27989-1-PBS)

    IP result of anti-SPEF2 (IP:27989-1-AP, 4ug; Detection:27989-1-AP 1:1000) with rat testis tissue lysate 1800 ug. This data was developed using the same antibody clone with 27989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SPEF2 Polyclonal antibody (27989-1-AP)

    IF Staining of mouse testis using 27989-1-AP (same clone as 27989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SPEF2 antibody (27989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27989-1-PBS in a different storage buffer formulation.

SPEF2 Polyclonal antibody, PBS Only

SPEF2 Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA
Cat No. 27989-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, Indirect ELISA

Synonyms

KPL2, Protein KPL2, sperm flagellar 2, Sperm flagellar protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27989-1-PBS targets SPEF2 in WB, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27506

Product name: Recombinant human SPEF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 103-316 aa of NM_024867

Sequence: MALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLM

相同性解析による交差性が予測される生物種
Full Name sperm flagellar 2
Calculated molecular weight 210 kDa
Observed molecular weight 210 kDa
GenBank accession numberNM_024867
Gene Symbol SPEF2
Gene ID (NCBI) 79925
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C093
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sperm flagellar protein 2 (SPEF2) is also named KPL2. As a cilia-associated protein, SPEF2 is broadly expressed in various cilia-related organs, such as the lung, spleen, trachea, brain, and testis (PMID: 10100999). SPEF2 is known to be essential for sperm tail development (PMID: 28619825). During spermatogenesis, SPEF2 is weakly expressed in cytoplasm in pachytene spermatocytes and round spermatocytes, then SPEF2 is localized in the manchette of steps 10-12 elongating spermatids, in the basal body at steps 13-14, and finally in the midpiece of the sperm tail at steps 14-16 (PMID: 19889948). Spef2 is expressed in the bone and cartilage, and Spef2 expression in osteoblasts increases during in vitro differentiation (PMID: 29339787).

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