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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using SPDYC Polyclonal antibody (22471-1-AP)

    IHC staining of human liver using 22471-1-AP (same clone as 22471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SPDYC Polyclonal antibody (22471-1-AP)

    IHC staining of human liver using 22471-1-AP (same clone as 22471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPDYC Polyclonal antibody (22471-1-AP)

    IHC staining of human kidney using 22471-1-AP (same clone as 22471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPDYC Polyclonal antibody (22471-1-AP)

    IHC staining of human kidney using 22471-1-AP (same clone as 22471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPDYC Polyclonal antibody (22471-1-AP)

    IHC staining of human lung cancer using 22471-1-AP (same clone as 22471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPDYC Polyclonal antibody (22471-1-AP)

    IHC staining of human lung cancer using 22471-1-AP (same clone as 22471-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22471-1-AP (SPDYC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22471-1-PBS in a different storage buffer formulation.

SPDYC Polyclonal antibody, PBS Only

SPDYC Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 22471-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Synonyms

hSpy/Ringo C, RINGO C, Ringo2, Speedy protein C

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22471-1-PBS targets SPDYC in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18126

Product name: Recombinant human SPDYC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-293 aa of BC137244

Sequence: MLWAIPELGSPCPISISYEMSDSQDPTTSPVVTTQVELGGCSRQGGGNGFLRFRQHQEVQAFLSLLEDSFVQEFLSKDPCFQISDKYLLAMVLVYFQRAHLKLSEYTHSSLFLALYLANDMEEDLEGPKCEIFPWALGKDWCLRVGKFLHQRDKLWARMGFRAVVSRQCCEEVMAKEPFHWAWTRDRRPHHGGVQRVCPQVPVRLPRGPGLSPPHCSPCGLPQHCSSHLLKPVSSKCPSLTSECHRPPSQNYLSRVKNAWGGDFLIVLPPQMQLEPGTYSLRIFPKPPARPGH

相同性解析による交差性が予測される生物種
Full Name speedy homolog C (Xenopus laevis)
Calculated molecular weight 293 aa, 33 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC137244
Gene Symbol SPDYC
Gene ID (NCBI) 387778
RRIDAB_3085699
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5MJ68
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPDYC(Speedy protein C), also known as RINGOC and Ringo2, promotes progression through the cell cycle via binding and activation of CDK1 and CDK2. Downregulation of SPDYC inhibits cell growth, reduces the fraction of cells in G1, and increases the S and G2 populations(PMID: 18802405). SPDYC is involved in the spindle-assembly checkpoint and required for recruitment of MAD2L1, BUBR1 and BUB1 to kinetochores(Uniprot).

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