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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using SPDYA Polyclonal antibody (17836-1-AP)

    WB analysis of Neuro-2a using 17836-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17836-1-AP (SPDYA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SPDYA Polyclonal antibody (17836-1-AP)

    IP experiment of mouse testis using 17836-1-AP

    IP result of anti-SPDYA (IP:17836-1-AP, 3ug; Detection:17836-1-AP 1:300) with mouse testis tissue lysate 4000ug.

SPDYA Polyclonal antibody

SPDYA Polyclonal Antibody for WB, IP, ELISA
Cat No. 17836-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNeuro-2a cells
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17836-1-AP targets SPDYA in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12041

Product name: Recombinant human SPDYA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC093005

Sequence: MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE

相同性解析による交差性が予測される生物種
Full Name speedy homolog A (Xenopus laevis)
Calculated molecular weight 313 aa, 36 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC093005
Gene Symbol SPDYA
Gene ID (NCBI) 245711
RRIDAB_2878448
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5MJ70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPDYA, also named as SPDY1, SPY1 and RINGO A, is a cell cycle regulator which regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP. It has two isoforms with MW 33 kDa and 36 kDa.

Protocols

Product Specific Protocols
WB protocol for SPDYA antibody 17836-1-APDownload protocol
IP protocol for SPDYA antibody 17836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols