Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, human skin tissue, PC-3 cells |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
Product Information
11847-1-AP targets SPCS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2407 Product name: Recombinant human SPCS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-102 aa of BC000884 Sequence: MLEHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLAQLTLPPWPIYRRHPLKWLPVQESSTDDKKPGERKIKRHAK 相同性解析による交差性が予測される生物種 |
| Full Name | signal peptidase complex subunit 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 102 aa, 12 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | BC000884 |
| Gene Symbol | SPCS1 |
| Gene ID (NCBI) | 28972 |
| RRID | AB_2195400 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6A9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SPCS1 antibody 11847-1-AP | Download protocol |
| WB protocol for SPCS1 antibody 11847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.
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PLoS Pathog Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.
| ||
Mol Cell Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway |
