Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, SKOV-3 cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87323-1-RR targets SPC25 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag23985 Product name: Recombinant human SPC25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-224 aa of BC022255 Sequence: MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN 相同性解析による交差性が予測される生物種 |
| Full Name | SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae) |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC022255 |
| Gene Symbol | SPC25 |
| Gene ID (NCBI) | 57405 |
| RRID | AB_3745470 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HBM1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Spindle Pole Body Component 25 (SPC25), a core subunit of the NDC80 kinetochore complex, is essential for accurate chromosome alignment and segregation during mitosis . By linking microtubules to kinetochores, SPC25 ensures proper attachment and tension generation, critical for the maintenance of genomic stability . SPC25 is largely found in the cytoplasm and nucleus. The NDC80 complex, which is involved in kinetochore-microtubule interactions and spindle assembly checkpoint activity, is composed of four subunits: SPC24, SPC25, NDC80, and NUF2. (PMID: 40587622 ,PMID: 40400636)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPC25 antibody 87323-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |