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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPATS2L Polyclonal antibody (29930-1-AP)

    WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SPATS2L Polyclonal antibody (29930-1-AP)

    WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATS2L Polyclonal antibody (29930-1-AP)

    IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATS2L Polyclonal antibody (29930-1-AP)

    IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATS2L Polyclonal antibody (29930-1-AP)

    IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

SPATS2L Polyclonal antibody, PBS Only

SPATS2L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29930-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29930-1-PBS targets SPATS2L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31157

Product name: Recombinant human SPATS2L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 290-488 aa of BC018736

Sequence: NYSSRTPCSSLLPLLNAHAATSGKQSNFSRKSSTHNKPSEGKAANPKMVSSLPSTADPSHQTMPANKQNGSSNQRRRFNPQYHNNRLNGPAKSQGSGNEAEPLGKGNSRHEHRRQPHNGFRPKNKGGAKNQEASLGMKTPEAPAHSEKPRRRQHAADTSEARPFRGSVGRVSQCNLCPTRIEVSTDAAVLSVPAVTLVA

相同性解析による交差性が予測される生物種
Full Name viral DNA polymerase-transactivated protein 6
Calculated molecular weight 488 aa, 54 kDa
Observed molecular weight54-65 kDa
GenBank accession numberBC018736
Gene Symbol SPATS2L
Gene ID (NCBI) 26010
RRIDAB_2923620
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUQ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

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