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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPATS2 Polyclonal antibody (32517-1-AP)

    WB analysis using 32517-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 32517-1-AP (SPATS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SPATS2 Polyclonal antibody (32517-1-AP)

    IP experiment of HeLa using 32517-1-AP

    IP result of anti-SPATS2 (IP:32517-1-AP, 4ug; Detection:32517-1-AP 1:5000) with HeLa cells lysate 1400 ug.

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SPATS2 Polyclonal antibody

SPATS2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32517-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Nbla00526, p59scr, SCR59, Serine-rich spermatocytes and round spermatid 59 kDa protein, SPATA10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32517-1-AP targets SPATS2 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPATS2 fusion protein Ag38507 相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated, serine-rich 2
Calculated molecular weight60kDa，545aa
Observed molecular weight70 kDa
GenBank accession numberNM_001293285.1
Gene Symbol SPATS2
Gene ID (NCBI) 65244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86XZ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPATS2, also known as SCR59 and SPATA10, is a member of the RNA-binding proteins whose aberrant expression is associated with carcinogenesis in several types of cancer. It is mainly expressed in the adult testis, with low expression in liver and other tissues (PMID: 33037180). The apparent molecular weight is higher than the calculated molecular weight of 60 kDa, probably due to post-translational modification, probably by phosphorylation.

Protocols

Product Specific Protocols
WB protocol for SPATS2 antibody 32517-1-APDownload protocol
IP protocol for SPATS2 antibody 32517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols