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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPATA5L1 Polyclonal antibody

SPATA5L1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18414-1-AP

Synonyms

SPATA5L1

Validation Data Gallery

Applications:
  • WB analysis using 18414-1-AP

    WB analysis using 18414-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18414-1-AP (SPATA5L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18414-1-AP

    WB analysis of HeLa using 18414-1-AP

    WB result of SPATA5L1 antibody (18414-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPATA5L1 transfected HeLa cells.

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

18414-1-AP targets SPATA5L1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPATA5L1 fusion protein Ag13269 相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 5-like 1
Calculated molecular weight 81 kDa
Observed molecular weight81 kDa
GenBank accession numberBC000981
Gene symbol SPATA5L1
Gene ID (NCBI) 79029
RRIDAB_2918062
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPATA5L1 (Spermatogenesis-associated protein 5-like protein 1) belongs to the AAA+ATPase protein superfamily. A full-length SPATA5L1 transcript and a short SPATA5L1 variant can be produced in human by alternative splicing. Bi-allelic SPATA5L1 variants lead to a human disease characterized by sensorineural hearing loss with or without a nonprogressive mixed neurodevelopmental phenotype. And immunofluorescence analysis in rat hippocampal neurons demonstrated that SPATA5L1 was located in neuronal and glial cell nuclei, with more prominent expression in neurons (PubMed:34626583). SPATA5L1 also has 3 isoforms.

Protocols

Product Specific Protocols
WB protocol for SPATA5L1 antibody 18414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols