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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    WB analysis of mouse testis using 16989-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPATA22 Polyclonal antibody (16989-1-AP)

    WB analysis using 16989-1-AP (same clone as 16989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    WB analysis of human testis using 16989-1-AP (same clone as 16989-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    IP experiment of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    IP result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SPATA22 Polyclonal antibody (16989-1-AP)

    IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPATA22 Polyclonal antibody, PBS Only

SPATA22 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16989-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

NYD SP20, SPATA 22, Spermatogenesis-associated protein 22, Testis development protein NYD-SP20

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16989-1-PBS targets SPATA22 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10591

Product name: Recombinant human SPATA22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-363 aa of BC029483

Sequence: MKRSLNENSARSTAGCLPVPLFNQKKRNRQPLTSNPLKDDSGISTPSDNYDFPPLPTDWAWEAVNPELAPVMKTVDTGQIPHSVSRPLRSQDSVFNSIQSNTGRSQGGWSYRDGNKNTSLKTWNKNDFKPQCKRTNLVANDGKNSCPMSSGAQQQKQLRTPEPPNLSRNKETELLRQTHSSKISGCTMRGLDKNSALQTLKPNFQQNQYKKQMLDDIPEDNTLKETSLYQLQFKEKASSLRIISAVIESMKYWREHAQKTVLLFEVLAVLDSAVTPGPYYSKTFLMRDGKNTLPCVFYEIDRELPRLIRGRVHRCVGNYDQKKNIFQCVSVRPASVSEQKTFQAFVKIADVEMQYYINVMNET

相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 22
Calculated molecular weight 363 aa, 41 kDa
Observed molecular weight 44, 41 kDa
GenBank accession numberBC029483
Gene Symbol SPATA22
Gene ID (NCBI) 84690
RRIDAB_2286640
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NHS9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.

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