Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31808-1-AP targets SPATA18 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36227 Product name: Recombinant human SPATA18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 102-332 aa of BC025396 Sequence: LQDTFDRERHKDPSPRDRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISLLAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQ 相同性解析による交差性が予測される生物種 |
| Full Name | spermatogenesis associated 18 homolog (rat) |
| Calculated molecular weight | 538 aa, 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC025396 |
| Gene Symbol | SPATA18 |
| Gene ID (NCBI) | 132671 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8TC71 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The modulation of mitophagy after DNA damage is independent of the type of DNA damage stimuli used and the protein spermatogenesis-associated protein 18 (SPATA18) is an important player in this process (PMID: 32453416; 21264221). SPATA18, also known as MIEAP, is a p53 transcriptional target and was previously reported to contribute to mitochondrial quality control by inducing intramitochondrial lysosome-like organelles after mitochondrial damage (PMID: 21264221).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPATA18 antibody 31808-1-AP | Download protocol |
| IHC protocol for SPATA18 antibody 31808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |