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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using SPARCL1 Polyclonal antibody (13517-1-AP)

    WB analysis of human heart using 13517-1-AP (same clone as 13517-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13517-1-AP (SPARCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Raji cells using SPARCL1 Polyclonal antibody (13517-1-AP)

    IP experiment of Raji using 13517-1-AP (same clone as 13517-1-PBS)

    IP result of anti-SPARCL1 (IP:13517-1-AP, 4ug; Detection:13517-1-AP 1:300) with Raji cells lysate 1440ug. This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SPARCL1 Polyclonal antibody (13517-1-AP)

    IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SPARCL1 Polyclonal antibody (13517-1-AP)

    IHC staining of human tonsillitis using 13517-1-AP (same clone as 13517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SPARCL1 Polyclonal antibody (13517-1-AP)

    IHC staining of human gliomas using 13517-1-AP (same clone as 13517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SPARCL1 Polyclonal antibody (13517-1-AP)

    IHC staining of human gliomas using 13517-1-AP (same clone as 13517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13517-1-PBS in a different storage buffer formulation.

SPARCL1 Polyclonal antibody, PBS Only

SPARCL1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13517-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13517-1-PBS targets SPARCL1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4448

Product name: Recombinant human SPARCL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 316-664 aa of BC033721

Sequence: VSEALLMEPTDDGNTTPRNHGVDDDGDDDGDDGGTDGPRHSASDDYFIPSQAFLEAERAQSIAYHLKIEEQREKVHENENIGTTEPGEHQEAKKAENSSNEEETSSEGNMRVHAVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPTCTDFEVIQFPLRMRDWLKNILMQLYEANSEHAGYLNEKQRNKVKKIYLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHWQFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

相同性解析による交差性が予測される生物種
Full Name SPARC-like 1 (hevin)
Calculated molecular weight 664 aa, 75 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC033721
Gene Symbol SPARCL1
Gene ID (NCBI) 8404
RRIDAB_10598475
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14515
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

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