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Validation Data Gallery

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  • Sandwich ELISA sample test of MP01430-2

    Sandwich ELISA sample test of MP01430-2

    Serum of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 212,811.0 pg/mL with a range of 153,761.1 - 333,381.8 pg/mL.

  • Sandwich ELISA sample test of MP01430-2

    Sandwich ELISA sample test of MP01430-2

    MG-63 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for human SPARC, and measured 91,240.8 pg/mL.

  • Sandwich ELISA sample test of MP01430-2

    Sandwich ELISA sample test of MP01430-2

    Human milk of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 1,084.0 pg/mL with a range of 425.0 - 1,594.2 pg/mL.

  • Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2, Human SPARC Recombinant Matched Antibody Pair - PBS only. 84621-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1169. 84621-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL

  • Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-3-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

SPARC Recombinant monoclonal antibody, PBS Only (Capture)

SPARC Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 84621-1-PBS
Clone No.241952A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, ON

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84621-1-PBS targets SPARC as part of a matched antibody pair:

MP01430-2: 84621-1-PBS capture and 84621-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1169

Product name: Recombinant Human SPARC protein (His Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 18-303 aa of NM_003118.4

Sequence: APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI

相同性解析による交差性が予測される生物種
Full Name secreted protein, acidic, cysteine-rich (osteonectin)
Calculated molecular weight35kDa
GenBank accession numberNM_003118.4
Gene Symbol SPARC
Gene ID (NCBI) 6678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09486
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.