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Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of DU 145 using 19527-1-AP (same clone as 19527-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of PC-3 using 19527-1-AP (same clone as 19527-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of HEK-293 using 19527-1-AP (same clone as 19527-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    WB analysis of PC-3 using 19527-1-AP (same clone as 19527-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human testis using 19527-1-AP (same clone as 19527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human skin cancer using 19527-1-AP (same clone as 19527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human skin cancer using 19527-1-AP (same clone as 19527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IHC staining of human testis using 19527-1-AP (same clone as 19527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19527-1-AP (SPANXA2/SPANXA1 antibody at dilution of 1:50. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPANXA2/SPANXA1 Polyclonal antibody (19527-1-AP)

    IF Staining of HEK-293 using 19527-1-AP (same clone as 19527-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 19527-1-AP (SPANXA2/SPANXA1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 19527-1-PBS in a different storage buffer formulation.

SPANXA2/SPANXA1 Polyclonal antibody, PBS Only

SPANXA2/SPANXA1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19527-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19527-1-PBS targets SPANXA2/SPANXA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name SPANX family, member A2
Calculated molecular weight 11 kDa
Observed molecular weight 27 kDa
GenBank accession numberNM_145662
Gene Symbol SPANXA2/SPANXA1
Gene ID (NCBI) 728712
RRIDAB_2878586
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NS26
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPANXA1, also named as SPANXA and SPANXA2, is a member of the SPANX family of cancer/testis-associated genes. It encodes differentially expressed testis-specific proteins that localize to various subcellular compartments. On Western blots of human testis proteins, SPANX antibodies reacts with the bands migrating between 15-30 kDa. The SPANX protein may be a useful target in cancer immunotherapy.

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