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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPAG8 Polyclonal antibody (13915-1-AP)

    WB analysis using 13915-1-AP (same clone as 13915-1-PBS)

    DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 13915-1-AP (SPAG8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SPAG8 Polyclonal antibody (13915-1-AP)

    IP experiment of mouse testis using 13915-1-AP (same clone as 13915-1-PBS)

    IP result of anti-SPAG8 (IP:13915-1-AP, 3ug; Detection:13915-1-AP 1:500) with mouse testis tissue lysate 6000ug. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SPAG8 Polyclonal antibody (13915-1-AP)

    IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SPAG8 Polyclonal antibody (13915-1-AP)

    IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SPAG8 Polyclonal antibody (13915-1-AP)

    IF Staining of PC-3 using 13915-1-AP (same clone as 13915-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SPAG8 antibody (13915-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

SPAG8 Polyclonal antibody, PBS Only

SPAG8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13915-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HSD-1, HSD 1, CT142, CILD28, BS84

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13915-1-PBS targets SPAG8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4910

Product name: Recombinant human SPAG8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC019247

Sequence: METNGSTEGSRSRSRSLDIQPSSEGLGPTSEPFPSSDDSPRSALAAATAAAAAAASAAAATAAFTTAKAAALSTKTPAPCSEFMEPSSDPSLLGEPCAGPGFTHNIAHGSLGFEPVYVSCIAQDTCTTTDHSSNPGPVPGSSSGPVLGSSSGAGHGSGSGSGPGCGSVPGSGSGPGPGSGPGSGPGHGSGSHPGPASGPGPDTGPDSELSPCIPPGFRNLVADRVLNYTSWSQHCPWEPQKQPPWEFLQVLEPGARGLWKPPDIKGKLMVCYETLPRGQCLLYNWEEERATNHLDQVPSMQ

相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 8
Calculated molecular weight 45 kDa, 54 kDa
Observed molecular weight 56-70 kDa
GenBank accession numberBC019247
Gene Symbol SPAG8
Gene ID (NCBI) 26206
RRIDAB_2194804
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99932
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPAG8, also named as Sperm-associated antigen 8 or HSD-1, is a 426 amino acid protein, which is expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. SPAG8 may play a role in fertility and microtubule formation through interaction with RANBP9. SPAG8 as testis-specific protein is produced during male germ cell differentiation and functions in a germ cell type- and stage-specific manner during spermatogenesis.

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