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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis of mouse ovary using 16294-1-AP (same clone as 16294-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis using 16294-1-AP (same clone as 16294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis of mouse brain using 16294-1-AP (same clone as 16294-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    WB analysis of mouse skeletal muscle using 16294-1-AP (same clone as 16294-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    IHC staining of human colon cancer using 16294-1-AP (same clone as 16294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPAG7 Polyclonal antibody (16294-1-AP)

    IHC staining of human colon cancer using 16294-1-AP (same clone as 16294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16294-1-PBS targets SPAG7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG7 fusion protein Ag9471 相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 7
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight 26-31 kDa
GenBank accession numberBC011934
Gene Symbol SPAG7
Gene ID (NCBI) 9552
RRIDAB_2302478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75391
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPAG7 encodes the sperm-associated antigen 7, which is widely expressed. The SPAG7 protein contains two nuclear localisation signals and a R3H domain. The domain is predicted to bind ssDNA or ssRNA in a sequence-specific manner. This might indicate a potential role of the protein in response to viruses or free DNAs or RNAs.